Article
Cloning, expression and functional analyses of the catabolite control protein CcpA from Bacillus megaterium.
Molecular microbiology - 1 Jun 1995
Hueck C J, Kraus A, Schmiedel D, Hillen W
Abstract excerpt
A mutant of Bacillus megaterium relieved from catabolite repression has been used to clone ccpA from B. megaterium by complementation. ccpA is the first gene of a presumed operon, in which it is followed by the motA homologue ORF1 and the motB homologue ORF2. The mutation maps in the 3'-terminal region of ccpA, where an in-frame duplication of 84 nucleotides located between two 9 bp direct repeats leads to an...
Topics
- Bacillus megaterium
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA-Binding Proteins
- Gene Library
- Genes, Bacterial
- Genetic Complementation Test
- Genotype
- Molecular Sequence Data
- Mutagenesis