Article
Morphological and functional characteristics of tumor-infiltrating lymphocytes from human colorectal cancers after stimulation with rIL-2.
European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes - 1 Jan 1995
Keller H, Wimmenauer S, Rahner S, Reimer P, von Kleist S, Farthmann E H
Abstract excerpt
Tumor-infiltrating lymphocytes (TILs) from colorectal cancers were separated from tumor cells by enzymatic and mechanical tissue disaggregation and discontinuous density gradients. Peripheral blood lymphocytes (PBLs) were isolated using the same procedure. The freshly separated TILs and PBLs were analyzed phenotypically by flow cytometry. The CD4+/CD8+ ratios of the freshly isolated TILs and PBLs were comparable...
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