Article
Role of porins in the antibiotic susceptibility of Pseudomonas aeruginosa: construction of mutants with deletions in the multiple porin genes.
Biochemical and biophysical research communications - 4 Aug 1995
Yoneyama H, Yamano Y, Nakae T
Abstract excerpt
We inserted deletions in the chromosomal genes of Pseudomonas aeruginosa coded for the outer membrane porins, proteins C, D2, or E1, and all possible combinations of these proteins by the gene replacement technique and selecting for imipenem-resistance. Determination of the minimum inhibitory con...
Topics
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Chromosome Mapping
- Chromosomes, Bacterial
- Cloning, Molecular
- Drug Resistance, Microbial
- Escherichia coli
- Gene Deletion
- Genes, Bacterial
- Phenotype
- Plasmids
- Porins
- Pseudomonas aeruginosa
- Restriction Mapping
- Tetracycline Resistance