Article
Purification and characterization of insulin-like growth factor II (IGF II) and an IGF II variant from human placenta.
Journal of chromatography. B, Biomedical applications - 21 Apr 1995
De Ceuninck F, Willeput J, Corvol M
Abstract excerpt
In order to purify variant IGF II peptides from human placenta, we have developed a purification procedure combining heparin affinity chromatography and cation-exchange, reversed-phase and size-exclusion HPLC. Two peptides were purified, both having apparent M(r) values of ca. 7300 Da as evaluate...
Topics
- Amino Acid Sequence
- Animals
- Chromatography, High Pressure Liquid
- Cross-Linking Reagents
- Female
- Genetic Variation
- Humans
- Insulin-Like Growth Factor I
- Insulin-Like Growth Factor II
- Molecular Sequence Data
- Placenta
- Pregnancy
- Swine