Article
Development of a shuttle vector for Moraxella catarrhalis.
Plasmid - 1 Jan 2006
Wang Wei, Attia Ahmed S, Liu Lixia, Rosche Thomas, Wagner Nikki J, Hansen Eric J
Abstract excerpt
Efforts to perform genetic analysis in Moraxella catarrhalis have been hampered by the lack of a cloning vector. M. catarrhalis strain E22 was previously shown to contain plasmid pLQ510 which lacked a selectable antibiotic resistance marker. Several methods were used to eliminate unnecessary DNA from pLQ510. Then, a 1.2 kb spectinomycin resistance cartridge, a multiple cloning site, and the origin of replication...
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