Microinjection of monoclonal antibody PAb421 into human SW480 colorectal carcinoma cells restores the transcription activation function to mutant p53.

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Article

Microinjection of monoclonal antibody PAb421 into human SW480 colorectal carcinoma cells restores the transcription activation function to mutant p53.

Cancer research - 15 Aug 1995

Abarzúa P, LoSardo J E, Gubler M L, Neri A

Abstract excerpt

The p53 tumor suppressor is a transcription factor frequently mutated in human malignancies. Tumor-derived p53 missense mutants are defective in sequence-specific DNA binding and fail to activate p53 target genes. mAb PAb421 was shown previously to restore DNA binding to selected p53 mutants in vitro. Here we show that mAb PAb421 when microinjected into human SW480 colorectal carcinoma cells restores the...

Topics

Antibodies, Monoclonal
Base Sequence
Binding Sites
Carcinoma
Colorectal Neoplasms
DNA-Binding Proteins
Gene Expression Regulation, Neoplastic
Genes, p53
In Vitro Techniques
Lung Neoplasms
Microinjections

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Oligodeoxyribonucleotides

RNA, Messenger

Transcription, Genetic

Transcriptional Activation

Tumor Cells, Cultured

Source

Open in PubMed

Identifiers

PMID: 7627952

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7627952-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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