Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA.

doi:10.1101/gad.6.7.1173

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Article

Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA.

Genes & development - 1 Jul 1992

Amberg D C, Goldstein A L, Cole C N

Abstract excerpt

We have combined techniques of genetics and histochemistry to identify genes required for the nucleocytoplasmic export of mRNA in the budding yeast Saccharomyces cerevisiae. We adapted in situ hybridization using a digoxigenin-labeled oligo(dT)50 probe to localize poly(A)+ RNA in fixed yeast cells and used yeast strains carrying the rna1-1 mutation to develop an assay. The rna1-1 mutation is the only previously...

Topics

Amino Acid Sequence
Base Sequence
Blotting, Northern
Cell Nucleus
Cloning, Molecular
Cytoplasm
DNA, Fungal
Exoribonucleases
Fungal Proteins
Genes, Fungal
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Nucleic Acid Hybridization

Phenotype

RNA, Fungal

RNA, Messenger

Restriction Mapping

Saccharomyces cerevisiae

Source

Open in PubMed

Identifiers

PMID: 1628825
DOI: 10.1101/gad.6.7.1173

Genes

XRN1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1628825-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA.PMID 1628825Read ↗

Dbp5p/Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export.Semantic proximity Read ↗

Mutation or deletion of the Saccharomyces cerevisiae RAT3/NUP133 gene causes temperature-dependent nuclear accumulation of poly(A)+ RNA and constitutive clustering of nuclear pore complexes.Semantic proximity Read ↗

Synthetic genetic array analysis in Saccharomyces cerevisiae provides evidence for an interaction between RAT8/DBP5 and genes encoding P-body components.Semantic proximity Read ↗

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