Article
Mutations causing high basal level transcription that is independent of transcriptional activators but dependent on chromosomal position in Saccharomyces cerevisiae.
Molecular & general genetics : MGG - 25 Jun 1995
Harashima S, Mizuno T, Mabuchi H, Yoshimitsu S, Ramesh R, Hasebe M, Tanaka A, Oshima Y
Abstract excerpt
Two single (bel2 and bel4) and two double (bel3 bel7 and bel5 be16) mutations causing enhanced transcription of a gene fusion, consisting of the open reading frame of PHO5 connected to the HIS5 promoter (HIS5p) integrated at the ura3 or leu2 locus, were isolated from a gcn4-disrupted mutant of Saccharomyces cerevisiae. The PHO5 gene, encoding repressible acid phosphatase, in the HIS5p-PHO5 construct was...
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