Characterization of FNR* mutant proteins indicates two distinct mechanisms for altering oxygen regulation of the Escherichia coli transcription factor FNR.

Abstract excerpt

In order to gain insight into the mechanism by which the Escherichia coli transcription factor FNR* is activated in response to anaerobiosis, we have analyzed FNR mutant proteins which, unlike the wild-type protein, stimulate gene expression in the presence of oxygen in vivo. Cell extracts containing seven different FNR* mutant proteins were tested in vitro for the ability to bind to the FNR consensus DNA site in...

Topics

• Aerobiosis
• Amino Acid Sequence
• Anaerobiosis
• Bacterial Proteins
• Cysteine
• DNA-Binding Proteins
• Escherichia coli
• Escherichia coli Proteins
• Gene Expression Regulation, Bacterial
• Iron-Sulfur Proteins