Article
Conversion of bacteriorhodopsin into a chloride ion pump.
Science (New York, N.Y.) - 7 Jul 1995
Sasaki J, Brown L S, Chon Y S, Kandori H, Maeda A, Needleman R, Lanyi J K
Abstract excerpt
In the light-driven proton pump bacteriorhodopsin, proton transfer from the retinal Schiff base to aspartate-85 is the crucial reaction of the transport cycle. In halorhodopsin, a light-driven chloride ion pump, the equivalent of residue 85 is threonine. When aspartate-85 was replaced with threonine, the mutated bacteriorhodopsin became a chloride ion pump when expressed in Halobacterium salinarium and, like...
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