Article
Protein-bound water as the determinant of asymmetric functional conversion between light-driven proton and chloride pumps.
Biochemistry - 12 Jun 2012
Muroda Kosuke, Nakashima Keisuke, Shibata Mikihiro, Demura Makoto, Kandori Hideki
Abstract excerpt
Bacteriorhodopsin (BR) and halorhodopsin (HR) are light-driven outward proton and inward chloride pumps, respectively. They have similar protein architecture, being composed of seven-transmembrane helices that bind an all-trans-retinal. BR can be converted into a chloride pump by a single amino acid replacement at position 85, suggesting that BR and HR share a common transport mechanism, and the ionic specificity...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Bacteriorhodopsins
- Chlorides
- Gene Expression Regulation, Bacterial
- Halorhodopsins
- Light
- Mutation
- Proton Pumps
- Spectroscopy, Fourier Transform Infrared