Constitutive forms of the enhancer-binding protein NtrC: evidence that essential oligomerization determinants lie in the central activation domain.

Abstract excerpt

Nitrogen regulatory protein C (NtrC) is a bacterial enhancer-binding protein that activates transcription by the sigma 54-holoenzyme. To activate transcription, NtrC must hydrolyze ATP, a reaction that depends upon its being phosphorylated and forming an appropriate oligomer. In this paper we characterize "constitutive" mutant forms of the NtrC protein from Salmonella typhimurium; unlike wild-type NtrC, these...

Topics

• Alleles
• Amino Acid Sequence
• Bacterial Proteins
• Base Sequence
• Binding Sites
• Cloning, Molecular
• DNA-Binding Proteins
• Enhancer Elements, Genetic
• Molecular Sequence Data
• Mutation
• PII Nitrogen Regulatory Proteins