Article
Glutamate at the site of phosphorylation of nitrogen-regulatory protein NTRC mimics aspartyl-phosphate and activates the protein.
Journal of molecular biology - 5 Jul 1993
Klose K E, Weiss D S, Kustu S
Abstract excerpt
The NTRC protein of enteric bacteria is an enhancer-binding protein that activates transcription by the sigma 54-holoenzyme form of RNA polymerase under nitrogen-limiting conditions. In vitro NTRC must be phosphorylated to catalyze ATP hydrolysis and activate transcription. The site of phosphoryl...
Topics
- Aspartic Acid
- Bacterial Proteins
- Base Sequence
- DNA-Binding Proteins
- Enhancer Elements, Genetic
- Gene Expression Regulation, Bacterial
- Glutamates
- Molecular Sequence Data
- Oligodeoxyribonucleotides
- PII Nitrogen Regulatory Proteins
- Phenotype
- Phosphoproteins
- Phosphorylation
- Promoter Regions, Genetic
- Protein Structure, Secondary
- Salmonella typhimurium
- Structure-Activity Relationship
- Trans-Activators