Article
Properties of a mutant form of the prokaryotic enhancer binding protein, NTRC, which hydrolyses ATP in the absence of effectors.
FEBS letters - 16 Oct 1998
Widdick D, Farez-Vidal E, Austin S, Dixon R
Abstract excerpt
The mutation S170A in the proposed nucleotide binding site of the transcriptional activator protein NTRC abolishes its ability to catalyse open promoter complex formation by the sigma(N)-RNA polymerase holoenzyme. NTRC(S170A) has significant ATPase activity, which, in contrast to the wild-type pr...
Topics
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Bacterial Proteins
- DNA-Binding Proteins
- Enhancer Elements, Genetic
- Glutamate-Ammonia Ligase
- Hydrolysis
- Mutation
- PII Nitrogen Regulatory Proteins
- Phosphorylation
- Protein Binding
- Trans-Activators
- Transcription Factors