Article
Influence of a mutation in the putative nucleotide binding site of the nitrogen regulatory protein NTRC on its positive control function.
Nucleic acids research - 11 May 1991
Austin S, Kundrot C, Dixon R
Abstract excerpt
A mutation, serine 170 to alanine, in the proposed ATP binding site of the activator protein NTRC prevents transcriptional activation at sigma 54-dependent promoters both in vivo and in vitro. The rate of phosphorylation of the mutant protein by NTRB and the stability of mutant NTRC-phosphate wer...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Bacterial Proteins
- Binding Sites
- DNA, Bacterial
- DNA-Binding Proteins
- Gene Expression Regulation
- Genes, Bacterial
- Klebsiella pneumoniae
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nitrogen Fixation
- PII Nitrogen Regulatory Proteins
- Phenotype
- Phosphorylation
- Promoter Regions, Genetic
- Sigma Factor