Article
Purification of cell populations from human fetal brain using flow cytometric techniques.
Brain research. Developmental brain research - 18 Apr 1995
Rozental R, Gebhard D, Padin C, Urban M, Wu J Y, Spray D C, Chiu F C
Abstract excerpt
We recently established primary cultures from dissociated second trimester human fetal brains using a novel spin seeding method and characterized cellular populations with distinct phenotypes in these cultures. Here, we report that these neural cultures can be dissociated to single-cell suspensions, sorted by size using flow cytometry and re-seeded to yield cultures selectively enriched for the neuronal and glial...
Topics
- Brain
- Cell Count
- Cell Separation
- Cells, Cultured
- Culture Techniques
- Electrophysiology
- Female
- Fetus
- Flow Cytometry
- Humans
- Immunohistochemistry