Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research

Abstract excerpt

BACKGROUND: Culturing primary cortical neurons is an essential neuroscience technique. However, most cultures are derived from rodent brains and standard protocols for human brain cultures are sparse. Herein, we describe preparation, maintenance and major characteristics of a primary human mixed brain culture, including neurons, obtained from legally aborted fetal brain tissue. This approach employs standard...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Alzheimer's disease research and treatments
• Share to Neurogenesis and neuroplasticity mechanisms
• Share to Pluripotent Stem Cells Research