Article
Maturation of murine natural killer precursor cells in the absence of exogenous cytokines requires contact with bone marrow stroma.
Natural immunity - 1 Jan 2000
Tsuji J M, Pollack S B
Abstract excerpt
To test whether differentiation of natural killer (NK) precursor cells in unsupplemented long-term bone marrow culture requires contact with stromal cells, bone marrow cells (BMC) that were depleted of NK 1.1+ and Thy-1+ cells were cultured either directly on established, irradiated bone marrow stroma or in cell culture inserts separated from the stroma by a 0.45-micron microporous membrane. No exogenous...
Topics
- Animals
- Bone Marrow
- Bone Marrow Cells
- Cell Adhesion
- Cell Communication
- Cell Differentiation
- Cells, Cultured
- Cytokines
- Cytotoxicity Tests, Immunologic
- Female
- Killer Cells, Natural