Article
The generation of natural killer (NK) cells from NK precursor cells in rat long-term bone marrow cultures.
The Journal of experimental medicine - 1 Jul 1990
van den Brink M R, Boggs S S, Herberman R B, Hiserodt J C
Abstract excerpt
In this report, we describe a novel long-term bone marrow culture (LTBMC) system to study the origin and generation of natural killer (NK) cells from NK precursors. Rat bone marrow was cultured for 4 wk in RPMI 1640 with 5% fetal calf serum and 2-mercaptoethanol to allow the formation of an adher...
Topics
- Animals
- Antibodies, Monoclonal
- Antibody-Dependent Cell Cytotoxicity
- Bone Marrow Cells
- Cell Count
- Cell Differentiation
- Cells, Cultured
- Flow Cytometry
- Hematopoietic Stem Cells
- Interleukin-2
- Killer Cells, Natural
- Male
- Mice
- Mice, Mutant Strains
- Phenotype
- Rats
- Rats, Inbred F344
- Recombinant Proteins