Article
Cloning and analysis of the leuB gene of Leptospira interrogans serovar pomona.
Journal of general microbiology - 1 May 1993
Ding M, Yelton D B
Abstract excerpt
The leuB gene of Leptospira interrogans serovar pomona strain kenniwicki has been cloned on a 9.5 kb plasmid, pWVL1, by complementation of Escherichia coli leuB mutants. Subcloning and Tn5 mutagenesis showed that the region required for complementation was approximately 1.2 kb in length. Enzyme assays showed that the product of the cloned gene was a beta-isopropylmalate dehydrogenase. Defects in the leuA, leuC...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
