Article
Reconstruction of glucose uptake and phosphorylation in a glucose-negative mutant of Escherichia coli by using Zymomonas mobilis genes encoding the glucose facilitator protein and glucokinase.
Journal of bacteriology - 1 Apr 1994
Snoep J L, Arfman N, Yomano L P, Fliege R K, Conway T, Ingram L O
Abstract excerpt
Expression of the Zymomonas mobilis glf (glucose facilitator protein) and glk (glucokinase) genes in Escherichia coli ZSC113 (glucose negative) provided a new functional pathway for glucose uptake and phosphorylation. Both genes were essential for the restoration of growth in glucose minimal medi...
Topics
- Bacterial Proteins
- Base Sequence
- Biological Transport
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Glucokinase
- Glucose
- Molecular Sequence Data
- Mutation
- Phosphorylation
- Zymomonas