Article
Targeted disruption of the tyrosine hydroxylase locus results in severe catecholamine depletion and perinatal lethality in mice.
The Journal of biological chemistry - 10 Nov 1995
Kobayashi K, Morita S, Sawada H, Mizuguchi T, Yamada K, Nagatsu I, Hata T, Watanabe Y, Fujita K, Nagatsu T
Abstract excerpt
Tyrosine 3-hydroxylase (TH, EC 1.14.16.2) catalyzes the first and rate-limiting step of the catecholamine biosynthetic pathway in the nervous and endocrine systems. The TH locus was disrupted in mouse embryonic stem cells by homologous recombination. Mice heterozygous for the TH mutation were apparently normal. In these mice, TH activity in the embryos and adult tissues was less than 50% of the wild-type values,...
Topics
- Animals
- Animals, Newborn
- Base Sequence
- Catecholamines
- DNA Primers
- Female
- Gene Targeting
- Genes, Lethal
- Heart
- Homozygote
- Humans