Article
Modular organization of T4 DNA polymerase. Evidence from phylogenetics.
The Journal of biological chemistry - 3 Nov 1995
Wang C C, Yeh L S, Karam J D
Abstract excerpt
We describe the use of a phylogenetic approach to analyze the modular organization of the single-chained (898 amino acids) and multifunctional DNA polymerase of phage T4. We have identified, cloned in expression vectors, and sequenced the DNA polymerase gene (gene 43) of phage RB69, a distant relative of T4. The deduced primary structure of the RB69 protein (RB69 gp43) differs from that of T4 gp43 in discrete...
Topics
- Amino Acid Sequence
- Bacteriophage T4
- Base Sequence
- Blotting, Southern
- DNA, Viral
- DNA-Directed DNA Polymerase
- Genes, Viral
- Genetic Variation
- Molecular Sequence Data
- Phylogeny
- Polymerase Chain Reaction