Article
Functional expression of the human CHIP28 water channel in a yeast secretory mutant.
FEBS letters - 16 Oct 1995
Laizé V, Rousselet G, Verbavatz J M, Berthonaud V, Gobin R, Roudier N, Abrami L, Ripoche P, Tacnet F
Abstract excerpt
The temperature-sensitive Saccharomyces cerevisiae mutant strain NY17, deficient in the secretory pathway (sec6-4 mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat-shock, the protein is present in partially purified post-golgi secretory vesicles. I...
Topics
- 4-Chloromercuribenzenesulfonate
- Aquaporin 1
- Aquaporins
- Base Sequence
- Blood Group Antigens
- Cytoplasmic Granules
- Fluorescent Antibody Technique
- Freeze Fracturing
- Humans
- Ion Channels
- Kinetics
- Microscopy, Electron
- Molecular Sequence Data
- Mutation
- Osmolar Concentration
- Recombinant Proteins
- Saccharomyces cerevisiae
- Sorbitol