Article
Functional expression of the human CHIP28 water channel in a yeast secretory mutant.
FEBS letters - 16 Oct 1995
Laizé V, Rousselet G, Verbavatz J M, Berthonaud V, Gobin R, Roudier N, Abrami L, Ripoche P, Tacnet F
Abstract excerpt
The temperature-sensitive Saccharomyces cerevisiae mutant strain NY17, deficient in the secretory pathway (sec6-4 mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat-shock, the protein is present in partially purified post-golgi secretory vesicles. Immunodetection and water transport studies, directly made on the vesicles, showed that CHIP28 is highly expressed and...
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