Article
Rapid protection against human immunodeficiency virus type 1 (HIV-1) replication mediated by high efficiency non-retroviral delivery of genes interfering with HIV-1 tat and gag.
Gene therapy - 1 Jan 1994
Lori F, Lisziewicz J, Smythe J, Cara A, Bunnag T A, Curiel D, Gallo R C
Abstract excerpt
Efficient transduction of inhibitory genes is a critical requirement in the development of a gene therapy strategy against human immunodeficiency virus type 1 (HIV-1). Commonly used systems based on retrovirus-mediated gene delivery are characterized by low efficiency gene transfer into the target cell. Genes were transduced in the absence of cell selection into 60-90% of human CD4+ cells by using a novel...
Topics
- Adenoviridae
- DNA, Recombinant
- Gene Transfer Techniques
- Genes, gag
- Genes, tat
- Genetic Therapy
- HIV-1
- HeLa Cells
- Humans
- Mutation
- Polylysine