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Allele-specific competitive blocker-PCR detection of rare base substitution.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2005

Parsons Barbara L, McKinzie Page B, Heflich Robert H

Abstract excerpt

Methods that detect rare base substitutions within populations of DNA molecules are valuable tools for studying the DNA-damaging effects of chemicals and for pool screening for single-nucleotide polymorphisms. Allele-specific competitive blocker-polymerase chain reaction (ACB-PCR) uses a mutant-specific PCR primer with more 3'-terminal mismatches to an abundant or wild-type sequence than to a rare or mutant...

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PMID: 15502227
DOI: 10.1385/1-59259-840-4:235

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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