Article
Identification, characterization and regional distribution in brain of RPDE-6 (RNPDE4A5), a novel splice variant of the PDE4A cyclic AMP phosphodiesterase family.
The Biochemical journal - 15 Sept 1995
McPhee I, Pooley L, Lobban M, Bolger G, Houslay M D
Abstract excerpt
COS-7 cells were transfected with a plasmid encoding a putative splice variant of PDE4A cyclic AMP-specific phosphodiesterase, RPDE-6 (RNPDE4A5). This led to the expression of a novel, cyclic AMP-specific, rolipram-inhibited phosphodiesterase activity. In such transfected cells a novel approximately 109 kDa species was recognized by anti-peptide sera raised against a dodecapeptide whose sequence is found at the...
Topics
- 3',5'-Cyclic-AMP Phosphodiesterases
- Alternative Splicing
- Amino Acid Sequence
- Animals
- Brain
- Cell Line
- Cell Membrane
- Chlorocebus aethiops
- Cyclic Nucleotide Phosphodiesterases, Type 4
- Cytosol