Article
Recombinant expression of a type IV, cAMP-specific phosphodiesterase: characterization and structure-function studies of deletion mutants.
Biochemistry - 11 Mar 1997
Kovala T, Sanwal B D, Ball E H
Abstract excerpt
A potential role for cAMP in regulating the differentiation of myoblasts has led us to examine the components of the cAMP signaling system, including the type IV, cAMP-specific phosphodiesterases. The full coding sequence of the phosphodiesterase PDE4D1 was inserted in the bacterial expression ve...
Topics
- 3',5'-Cyclic-AMP Phosphodiesterases
- Animals
- Cations, Divalent
- Cell Line
- Chromatography, Gel
- Cloning, Molecular
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression
- Kinetics
- Muscle Fibers, Skeletal
- Mutation
- Recombinant Fusion Proteins
- Sequence Deletion
- Structure-Activity Relationship