Article
Identification of two splice variant forms of type-IVB cyclic AMP phosphodiesterase, DPD (rPDE-IVB1) and PDE-4 (rPDE-IVB2) in brain: selective localization in membrane and cytosolic compartments and differential expression in various brain regions.
The Biochemical journal - 1 Dec 1994
Lobban M, Shakur Y, Beattie J, Houslay M D
Abstract excerpt
In order to detect the two splice variant forms of type-IVB cyclic AMP phosphodiesterase (PDE) activity, DPD (type-IVB1) and PDE-4 (type-IVB2), anti-peptide antisera were generated. One set ('DPD/PDE-4-common'), generated against a peptide sequence found at the common C-terminus of these two PDEs...
Topics
- 3',5'-Cyclic-AMP Phosphodiesterases
- Amino Acid Sequence
- Animals
- Brain
- Cell Line
- Cell Membrane
- Cerebral Cortex
- Cytosol
- Gene Expression
- Genetic Variation
- Male
- Molecular Sequence Data
- RNA Splicing
- Rats
- Rats, Sprague-Dawley