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Article

Correlation of two-hybrid affinity data with in vitro measurements.

Molecular and cellular biology - 1 Oct 1995

Estojak J, Brent R, Golemis E A

Abstract excerpt

Since their introduction, the interaction trap and other two-hybrid systems have been used to study protein-protein interactions. Despite their general use, little is known about the extent to which the degree of protein interaction determined by two-hybrid approaches parallels the degree of interaction determined by biochemical techniques. In this study, we used a set of lexAop-LEU2 and lexAop-lacZ reporters to...

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Identifiers

PMID: 7565735
DOI: 10.1128/MCB.15.10.5820
PMCID: PMC230834

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