Article
Correlation of two-hybrid affinity data with in vitro measurements.
Molecular and cellular biology - 1 Oct 1995
Estojak J, Brent R, Golemis E A
Abstract excerpt
Since their introduction, the interaction trap and other two-hybrid systems have been used to study protein-protein interactions. Despite their general use, little is known about the extent to which the degree of protein interaction determined by two-hybrid approaches parallels the degree of inte...
Topics
- Bacterial Proteins
- Base Sequence
- DNA
- DNA-Binding Proteins
- Genes, Reporter
- Helix-Loop-Helix Motifs
- Kinetics
- Leucine
- Molecular Sequence Data
- Mutation
- Operator Regions, Genetic
- Protein Binding
- Recombinant Fusion Proteins
- Repressor Proteins
- Saccharomyces cerevisiae
- Serine Endopeptidases
- Transcriptional Activation
- Viral Proteins