Article
Serologic subtyping of HLA-DR8 by means of the cytotoxic human monoclonal antibody 5643.
Human immunology - 1 Jul 1995
Viken H D, Thoresen A B, Knutsen I, Thorsby E, Hansen T
Abstract excerpt
EBV-transformed B cells from a multiparous woman with anti-HLA antibodies were hybridized with CB-F7 heteromyeloma cells. The resulting hybridoma produced the cytotoxic human IgM(kappa) mAb 5643. Testing of the hybridoma supernatant against HLA homozygous cell lines demonstrated positive reactions (titer range 128-512) only with three cell lines that carried the DR (alpha, beta 1*0801) subtype of DR8. There was...
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