Article
Dissecting the role of protein-protein and protein-nucleic acid interactions in MS2 bacteriophage stability.
The FEBS journal - 1 Apr 2006
Lima Sheila M B, Vaz Ana Carolina Q, Souza Theo L F, Peabody David S, Silva Jerson L, Oliveira Andréa C
Abstract excerpt
To investigate the role of protein-protein and protein-nucleic acid interactions in virus assembly, we compared the stabilities of native bacteriophage MS2, virus-like particles (VLPs) containing nonviral RNAs, and an assembly-defective coat protein mutant (dlFG) and its single-chain variant (sc-dlFG). Physical (high pressure) and chemical (urea and guanidine hydrochloride) agents were used to promote virus...
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