Article
Mutations that increase the affinity of a translational repressor for RNA.
Nucleic acids research - 11 Sept 1994
Lim F, Peabody D S
Abstract excerpt
The coat protein of the RNA bacteriophage MS2 is a specific RNA binding protein that represses translation of the viral replicase gene during the infection cycle. As an approach to characterizing the RNA-binding site of coat protein we have isolated a series of coat mutants that suppress the effects of a mutation in the translational operator. Each of the mutants exhibits a super-repressor phenotype, more tightly...
Topics
- Base Sequence
- Capsid
- Kinetics
- Levivirus
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Protein Biosynthesis
- RNA, Viral
- RNA-Binding Proteins
- RNA-Dependent RNA Polymerase