Article
Binding in vitro of phosphotyrosine-containing proteins to pp60c-src SH2 domain does not correlate with CEF transformation.
Oncogene - 2 Feb 1995
Boeuf H, Murphy J, Bibbins K B, Varmus H E
Abstract excerpt
We have previously described pp60c-src SH2 mutants that are host-range-dependent for cell transformation; most of these mutants can transform CEF cells but not NIH3T3 cells, and other transform NIH3T3 cells more efficiently than CEF (Hirai and Varmus, 1990c). In an attempt to understand the molecular basis of these phenotypes, we analysed the ability of mutant SH2 domains in GST fusion proteins to bind to...
Topics
- 3T3 Cells
- Animals
- Cell Line
- Cell Transformation, Neoplastic
- Chick Embryo
- Fibroblasts
- Mice
- Mutation
- Phosphoproteins
- Phosphotyrosine
- Protein Binding
- Proto-Oncogene Proteins pp60(c-src)