Article
Spontaneous dissociation-association of monomers of the human-stem-cell-factor dimer.
The Biochemical journal - 15 Jan 1995
Lu H S, Chang W C, Mendiaz E A, Mann M B, Langley K E, Hsu Y R
Abstract excerpt
In its native state, recombinant human-stem-cell-factor (SCF) dimer can spontaneously and rapidly undergo hybridization when two different SCF dimer species are incubated together. SCF species differing in molecular charge, e.g., a wild-type SCF form and a variant with Asp at position 10 instead of Asn, were used in the hybridization studies; the original species and newly formed dimer hybrid can be separated and...
Topics
- Amino Acid Sequence
- Escherichia coli
- Glycoproteins
- Hematopoietic Cell Growth Factors
- Humans
- Hydrogen-Ion Concentration
- Models, Chemical
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Recombinant Proteins