Article
Cloning and sequence of a functionally active cDNA encoding the mouse ubiquitin-activating enzyme E1.
Gene - 10 Sept 1992
Imai N, Kaneda S, Nagai Y, Seno T, Ayusawa D, Hanaoka F, Yamao F
Abstract excerpt
A cDNA encoding the ubiquitin-activating enzyme, E1, was isolated from the mouse mammary carcinoma cell line, FM3A, and shown to complement mutant mouse cells deficient in the enzyme. The 3495-bp cDNA encodes 1058 amino acids (aa), and shares extensive homology with the human E1 enzyme at both the nucleotide and aa sequence levels.
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Cloning, Molecular
- DNA
- Ligases
- Mice
- Molecular Sequence Data
- Mutation
- Sequence Homology, Nucleic Acid
- Ubiquitin-Activating Enzymes