Expression of rat alpha-fetoprotein cDNA and its mutants in cultured mouse fibroblasts and identification of glycosylation sites related to electrophoretic variants.

doi:10.1016/0167-4838(94)90121-x

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Article

Expression of rat alpha-fetoprotein cDNA and its mutants in cultured mouse fibroblasts and identification of glycosylation sites related to electrophoretic variants.

Biochimica et biophysica acta - 19 Oct 1994

Koyama Y, Nishi S

Abstract excerpt

Rat alpha-fetoprotein (RAFP) shows two discrete electrophoretic variants, slow and fast, with molecular weights of 72,500 and 69,500, respectively. To elucidate the structural basis of this heterogeneity, we developed the expression system of RAFP cDNA and its mutants with the use of cultured mou...

Topics

Amidohydrolases
Amino Acid Sequence
Animals
Base Sequence
Binding Sites
Cell Line
DNA, Complementary
Electrophoresis, Polyacrylamide Gel
Genetic Vectors
Glycosylation
Mice
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase

Polymerase Chain Reaction

Rats

Recombinant Proteins

alpha-Fetoproteins

Source

Open in PubMed

Identifiers

PMID: 7524678
DOI: 10.1016/0167-4838(94)90121-x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7524678-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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