Article
Novel culture procedure permitting the synthesis of proteins by rat calvarial cells cultured on hydroxyapatite particles to be quantified.
Biomaterials - 1 Jun 1994
Sammons R L, el Haj A J, Marquis P M
Abstract excerpt
A simple culture procedure and assay conditions are described which have permitted us to quantify the synthesis of proteins which are associated with an osteoblastic phenotype, by rat calvarial periosteal cells grown on particulate materials. The main feature of the method is the use of an adhesi...
Topics
- Alkaline Phosphatase
- Animals
- Biocompatible Materials
- Blotting, Western
- Cell Adhesion
- Cell Division
- Cells, Cultured
- Collagen
- Durapatite
- Electrophoresis, Polyacrylamide Gel
- Luminescent Measurements
- Microscopy, Electron, Scanning
- Molecular Weight
- Osteoblasts
- Osteopontin
- Periosteum
- Phenotype
- Rats