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Article

Typing of Clostridium difficile strains by PCR-amplification of variable length 16S-23S rDNA spacer regions.

Journal of general microbiology - 1 Dec 1993

Gürtler V

Abstract excerpt

To develop a rapid and accurate method of typing large numbers of clinical isolates of Clostridium difficile, four regions of the rRNA operon [A, 15-1407 and B, 907-1407 (16S-16S); C, 1392-507 and D, 907-507 (16S-23S)] were enzymically amplified from 24 strains. When region A was hybridized to HindIII-digested genomic DNA isolated from C. difficile strains, all of the variable length restriction fragments...

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Identifiers

PMID: 7510324
DOI: 10.1099/00221287-139-12-3089

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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