Typing of Clostridium difficile strains by PCR-amplification of variable length 16S-23S rDNA spacer regions.

doi:10.1099/00221287-139-12-3089

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Article

Typing of Clostridium difficile strains by PCR-amplification of variable length 16S-23S rDNA spacer regions.

Journal of general microbiology - 1 Dec 1993

Gürtler V

Abstract excerpt

To develop a rapid and accurate method of typing large numbers of clinical isolates of Clostridium difficile, four regions of the rRNA operon [A, 15-1407 and B, 907-1407 (16S-16S); C, 1392-507 and D, 907-507 (16S-23S)] were enzymically amplified from 24 strains. When region A was hybridized to Hi...

Topics

Bacterial Typing Techniques
Base Sequence
Clostridioides difficile
DNA Primers
DNA, Bacterial
DNA, Ribosomal
Genetic Variation
Humans
Molecular Sequence Data
Operon
Polymerase Chain Reaction
RNA, Bacterial
RNA, Ribosomal, 16S
RNA, Ribosomal, 23S

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