Typing of Staphylococcus aureus strains by PCR-amplification of variable-length 16S-23S rDNA spacer regions: characterization of spacer sequences.

doi:10.1099/13500872-141-5-1255

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Article

Typing of Staphylococcus aureus strains by PCR-amplification of variable-length 16S-23S rDNA spacer regions: characterization of spacer sequences.

Microbiology (Reading, England) - 1 May 1995

Grtler Volker, Barrie Helen D

Abstract excerpt

To develop a rapid and accurate method of typing large numbers of clinical isolates of Staphylococcus aureus, the spacer region C of the rRNA operon [1391-507 (16S-23S)] was enzymically amplified from 322 strains. When the products were separated by denaturing PAGE, 15 variable-length rrn alleles were demonstrated, ranging in size from 906 to 1223 bp. The variable-length HpaII-digested region C [(region E;...

Topics

Alleles
Base Sequence
DNA Primers
DNA, Ribosomal
Genes, Bacterial
Humans
Methicillin Resistance
Molecular Sequence Data
Penicillin Resistance
Phylogeny
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA, Ribosomal, 16S

RNA, Ribosomal, 23S

RNA, Transfer, Phe

Reproducibility of Results

Restriction Mapping

Sequence Homology, Nucleic Acid

Staphylococcus aureus

Source

Open in PubMed

Identifiers

PMID: 7773419
DOI: 10.1099/13500872-141-5-1255

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7773419-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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