Article
Classification of medically important clostridia using restriction endonuclease site differences of PCR-amplified 16S rDNA.
Journal of general microbiology - 1 Nov 1991
Gurtler V, Wilson V A, Mayall B C
Abstract excerpt
Restriction maps were constructed of enzymically amplified 16S rRNA genes (rDNA) isolated from eight Clostridium species. Using maximum parsimony, a dendrogram was constructed from these and published 16S rRNA sequence data. Two distinct clusters were identified: cluster I contained C. difficile, C. sordelli, and C. bifermentans, and showed 30 of 35 restriction sites in common; cluster II contained C. tetani, C....
Topics
- Alleles
- Base Sequence
- Blotting, Southern
- Clostridium
- DNA, Bacterial
- DNA, Ribosomal
- Electrophoresis, Gel, Pulsed-Field
- Molecular Sequence Data
- Phylogeny
- Polymerase Chain Reaction
- RNA, Ribosomal, 16S