PCR-amplified 16S and 23S rDNA restriction analysis for the identification of Acinetobacter strains at the DNA group level.

doi:10.1016/s0923-2508(97)82453-9

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Article

PCR-amplified 16S and 23S rDNA restriction analysis for the identification of Acinetobacter strains at the DNA group level.

Research in microbiology - 1 Dec 1997

García-Arata M I, Gerner-Smidt P, Baquero F, Ibrahim A

Abstract excerpt

The genus Acinetobacter is phenotypically rather homogeneous, but genotypically heterogeneous. In this study, a simple method based on restriction analysis of a PCR-amplified large fragment (4.5 kb) of most of the ribosomal operon (16S and 23S ribosomal genes and the spacer in-between) was invest...

Topics

Acinetobacter
DNA, Bacterial
Deoxyribonucleases, Type II Site-Specific
Genotype
Polymerase Chain Reaction
RNA, Ribosomal, 16S
RNA, Ribosomal, 23S
Restriction Mapping
Templates, Genetic

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