Article
Nicotine regulates nicotinic cholinergic receptors and subunit mRNAs in PC 12 cells through protein kinase A.
Brain research. Molecular brain research - 1 Aug 1995
Madhok T C, Matta S G, Sharp B M
Abstract excerpt
To understand the up-regulation of neuronal nicotinic cholinergic receptors (nAcChRs) that results from chronic in vivo treatment with nicotine, we studied the effect of nicotine on [3H]nicotine binding sites on PC 12 cells. PC 12 cells were grown in nicotine hemisulfate (10(-6) to 10(-3) M) or vehicle for 7 days, and specific [3H]nicotine binding was measured. Nicotine (10(-6) to 10(-4) M) dose-dependently...
Topics
- Animals
- Blotting, Northern
- Cyclic AMP-Dependent Protein Kinases
- Mutation
- Neurons
- Nicotine
- PC12 Cells
- RNA, Messenger
- Rats
- Receptors, Nicotinic
- Statistics as Topic