Article
The SOS chromotest: direct assay of the expression of gene sfiA as a measure of genotoxicity of chemicals.
Biochimie - 1 Jan 2000
Quillardet P, Huisman O, D'Ari R, Hofnung M
Abstract excerpt
We used a gene fusion, placing the lacZ gene encoding beta-galactosidase under the control of the sfiA promoter, to construct a new tester strain for genotoxic agents. The assay is performed in a few hours and involves simple enzymatic assays. The dose response curves contain a linear portion which enables to define the SOS Inducing Potency (SOSIP) of compounds. For the compounds tested SOSIPs extend over 7...
Topics
- 4-Nitroquinoline-1-oxide
- Animals
- Biotransformation
- DNA Repair
- Genes
- Microsomes, Liver
- Mutagenicity Tests
- Mutagens
- Mutation
- Operon
- Salmonella typhimurium
- beta-Galactosidase