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Article

SOS induction as an in vivo assay of enzyme-DNA interactions.

Gene - 15 Jul 1991

Heitman J, Model P

Abstract excerpt

We have constructed strains which are convenient and sensitive indicators of DNA damage and describe their use. These strains utilize an SOS::lac Z fusion constructed by Kenyon and Walker [Proc. Natl. Acad. Sci. USA 77 (1980) 2819-2823] and respond to DNA damage by producing beta-galactosidase. They can be used to characterize restriction systems and screen for restriction endonuclease mutants. Applications...

Topics

DNA
DNA Damage
DNA Modification Methylases
DNA-Binding Proteins
Deoxyribonuclease EcoRI
Escherichia coli
Gene Expression Regulation, Bacterial
Mutation
Recombinant Fusion Proteins
SOS Response, Genetics
beta-Galactosidase

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Source

Identifiers

PMID: 1908806
DOI: 10.1016/0378-1119(91)90383-m

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1908806-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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