Article
An improved assay for the detection of alterations in bacterial DNA supercoiling in vivo.
Die Pharmazie - 1 Jul 2013
Abu Mraheil M, Heisig A, Heisig P
Abstract excerpt
Due to the increasing prevalence of antibiotic resistance and the yet low output of the genomics-based drug discovery approach novel strategies are urgently needed to detect new antibiotics. One such strategy uses known ubiquitous targets like DNA topoisomerases. However, to detect inhibitors of these enzymes by an in vitro assay time-consuming isolation of enzymes and DNA followed by electrophoretic separation...
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