Absence of pyrimidine insertase activity in E. coli extracts, using plasmid DNA containing apyrimidinic sites.

doi:10.1016/0167-8817(84)90002-6

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Article

Absence of pyrimidine insertase activity in E. coli extracts, using plasmid DNA containing apyrimidinic sites.

Mutation research - 1 Jan 2000

Martin B, Sicard N

Abstract excerpt

Plasmid DNA, isolated from mutants of E. coli that are deficient in both uracil-DNA glycosylase and deoxyuridine triphosphatase, contains significant amounts of uracil. This can be removed in vitro by the action of uracil-DNA glycosylase, creating apyrimidinic sites. We have used depyrimidinated...

Topics

DNA Repair
DNA Replication
Escherichia coli
Genotype
Mutation
Plasmids
Polynucleotides
Species Specificity
Thymine Nucleotides

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