Article
Endonuclease A degrades chromosomal and plasmid DNA of Escherichia coli present in most preparations of single stranded DNA from phagemids.
Proceedings of the National Science Council, Republic of China. Part B, Life sciences - 1 Jan 1992
Lin J J
Abstract excerpt
With E. coli, large and variable amounts of chromosomal and plasmid DNAs are observed in the supernatants of overnight cultures when the cells carry an endA mutation, but are not detected by gel electrophoresis when the cells carry the wild type allele of endA. Significant amounts of nuclease activity in DH11S endA+ supernatants were detected by two simple assays; the rapid degradation of added pBR322 plasmid...
Topics
- Bacteriophages
- DNA, Bacterial
- DNA, Single-Stranded
- DNA, Viral
- Deoxyribonucleases
- Escherichia coli
- Genetic Vectors
- Mutation
- Plasmids
- Substrate Specificity