Article
Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria.
Microbiology (Reading, England) - 1 Aug 2001
Marx Christopher J, Lidstrom Mary E
Abstract excerpt
Full exploitation of the information available in bacterial genome sequences requires the availability of facile tools for rapid genetic manipulation. One bacterium for which new genetic tools are needed is the methylotroph Methylobacterium extorquens AM1. IncQ and small IncP vectors were shown to be unsuitable for use in this bacterium, but a spontaneous mutant of a small IncP plasmid was isolated that...
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