Article
[Isolation and characteristics of a temperature-sensitive plasmid pRP19.6--an RP1 derivative containing the duplicated IS21 sequence].
Molekuliarnaia biologiia - 1 Jan 2000
Danilevich V N, Kostiuchenko D A, Negriĭ N V, Shtannikov A V
Abstract excerpt
When analysing the antibiotic resistant, temperature-independent derivatives of Proteus mirabilis cells, carrying the plasmid RP1ts12, a derivative of the latter (pRP19.6) with an elevated frequency of integration into E. coli K12 chromosome, has been isolated. The structure and properties of pRP19.6 was studied. As revealed from the data of structural and genetic analyses pRP19.6 is identical to the factor...
Topics
- Chromosome Mapping
- Chromosomes, Bacterial
- Cloning, Molecular
- Conjugation, Genetic
- DNA Restriction Enzymes
- Escherichia coli
- Genetic Markers
- Mutation
- Phenotype
- Plasmids
- Proteus mirabilis
